monoclonal mouse anti-human notch1 Search Results


monoclonal mouse anti-human notch1  (BioCarta)
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BioCarta monoclonal mouse anti-human notch1
Cellular architecture and proliferative capacity of the adult human SVZ. a, Schematic overview of a human brain hemisphere with the dotted red line indicating the area of analysis. b, c, Representative histology of the SVZ with a gap between the ependymal layer and astrocytic ribbon in the medial part (b) and with the astrocytic ribbon being continuous from the ependymal layer to the brain parenchyma in the dorsal and ventral areas (c). d, Occasionally, PSA-NCAM-positive cells can be detected close to the ependyma or in the astrocytic ribbon and beyond. e, f, Molecules that were shown to regulate neurogenesis such as <t>notch1</t> (e) and Flk-1/VEGFR-2 (f) are expressed in the ependymal cell layer. g, Proliferating Ki-67-positive cells are often closely associated with the ependymal layer. h, i, Lack of costaining with pan-hematopoietic markers such as CD45 (h) rules out a non-neural origin of the proliferating Ki-67-positive cells (i). Scale bars: b, c, 50 μm; d, g, h, 10 μm; e, f, 25 μm; i, 20 μm.
Monoclonal Mouse Anti Human Notch1, supplied by BioCarta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cellular architecture and proliferative capacity of the adult human SVZ. a, Schematic overview of a human brain hemisphere with the dotted red line indicating the area of analysis. b, c, Representative histology of the SVZ with a gap between the ependymal layer and astrocytic ribbon in the medial part (b) and with the astrocytic ribbon being continuous from the ependymal layer to the brain parenchyma in the dorsal and ventral areas (c). d, Occasionally, PSA-NCAM-positive cells can be detected close to the ependyma or in the astrocytic ribbon and beyond. e, f, Molecules that were shown to regulate neurogenesis such as notch1 (e) and Flk-1/VEGFR-2 (f) are expressed in the ependymal cell layer. g, Proliferating Ki-67-positive cells are often closely associated with the ependymal layer. h, i, Lack of costaining with pan-hematopoietic markers such as CD45 (h) rules out a non-neural origin of the proliferating Ki-67-positive cells (i). Scale bars: b, c, 50 μm; d, g, h, 10 μm; e, f, 25 μm; i, 20 μm.

Journal: The Journal of Neuroscience

Article Title: Increased Generation of Neuronal Progenitors after Ischemic Injury in the Aged Adult Human Forebrain

doi: 10.1523/JNEUROSCI.4667-06.2006

Figure Lengend Snippet: Cellular architecture and proliferative capacity of the adult human SVZ. a, Schematic overview of a human brain hemisphere with the dotted red line indicating the area of analysis. b, c, Representative histology of the SVZ with a gap between the ependymal layer and astrocytic ribbon in the medial part (b) and with the astrocytic ribbon being continuous from the ependymal layer to the brain parenchyma in the dorsal and ventral areas (c). d, Occasionally, PSA-NCAM-positive cells can be detected close to the ependyma or in the astrocytic ribbon and beyond. e, f, Molecules that were shown to regulate neurogenesis such as notch1 (e) and Flk-1/VEGFR-2 (f) are expressed in the ependymal cell layer. g, Proliferating Ki-67-positive cells are often closely associated with the ependymal layer. h, i, Lack of costaining with pan-hematopoietic markers such as CD45 (h) rules out a non-neural origin of the proliferating Ki-67-positive cells (i). Scale bars: b, c, 50 μm; d, g, h, 10 μm; e, f, 25 μm; i, 20 μm.

Article Snippet: The following antibodies were used: mouse anti-β-III-tubulin (clone Tuj1 1:500; Covance), monoclonal mouse anti-polysialic acid neural cell adhesion molecule (PSA-NCAM, clone 2–2B, 1:2000; Millipore, Temecula, CA), monoclonal mouse anti-human notch1 (1:2000, Ab-1, clone A6; Biocarta Europe, Hamburg, Germany), polyclonal rabbit anti-vascular endothelial growth factor -receptor-2 (VEGFR2)/fetal liver kinase receptor 1 (Flk-1) (1:100; Zytomed, Berlin, Germany), monoclonal mouse anti-glial fibrillary acidic protein (GFAP, 1:500; Millipore), polyclonal rabbit anti-GFAP (1:1000; Dako, Carpinteria, CA), monoclonal mouse anti-human CD45/leukocyte common antigen (1:50, clones 2B11+PD7/26; Dako), monoclonal mouse anti-human CD68 (clone PG-M1, 1:100; Dako), monoclonal mouse anti-human CD34 (clone QBEND10, 1:100; Immunotech, Marseille, France), polyclonal rabbit anti-human S100β (1:100; Dako), monoclonal mouse anti-human nestin (1:200; Millipore), polyclonal rabbit anti-Mash1 (1:100; Millipore), polyclonal rabbit anti-Musashi (1:100; Millipore), and monoclonal mouse anti-human Ki-67 (clone MIB-1, 1:50; Dako).

Techniques: